Assessing the utility of seed coat-specific promoters to engineer cell wall polysaccharide composition of mucilage.

McGee R, Dean GH, Mansfield SD, Haughn GW
Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
128432	R4pGWB401+Citrine	Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.
128431	pDONR_P4-P1R:PER36modp	Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).
128429	pDONRG_P4-P1R:TBA2p	Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.