A Seed Mismatch Enhances Argonaute2-Catalyzed Cleavage and Partially Rescues Severely Impaired Cleavage Found in Fish.
Chen GR, Sive H, Bartel DP
Mol Cell. 2017 Dec 21;68(6):1095-1107.e5. doi: 10.1016/j.molcel.2017.11.032.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221710 | pCS2+-eGFP | Expression of eGFP in zebrafish embryos. |
| 221711 | pCS2+-drAgo2 | Expression of zebrafish Ago2 in zebrafish embryos. |
| 221712 | pCS2+-FLAG3-drAgo2 | Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. |
| 221713 | pCS2+-FLAG3-drAgo2D-A | Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221714 | pCS2+-FLAG3-drAgo2D-E | Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221715 | pCS2+-FLAG3-drAgo2Y-F | Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221716 | pCS2+-FLAG3-drAgo2DY-EF | Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221717 | pCS2+-FLAG3-hsN-drAgo2 | Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain. |
| 221718 | pCS2+-hsAgo2 | Expression of human Ago2 in zebrafish embryos. |
| 221719 | pCS2+-FLAG3-hsAgo2 | Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. |
| 221720 | pCS2+-FLAG3-hsAgo2D-A | Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221721 | pCS2+-FLAG3-hsAgo2E-D | Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221722 | pCS2+-FLAG3-hsAgo2F-Y | Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221723 | pCS2+-FLAG3-hsAgo2EF-DY | Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos. |
| 221724 | pCS2+-FLAG3-drN-hsAgo2 | Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain. |
| 221725 | pCS2+-Zeo | Expression of Zeocin resistance marker in zebrafish embryos. |
| 221726 | pCS2+-Zeo-miR-430 | Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos. |
| 221727 | pCS2+-Zeo-miR-430-10-11 mm | Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos. |
| 221728 | pCS2+-Zeo-miR-430-G-G mm | Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos. |
| 221730 | pcDNA3-FLAG3-drAgo2 | Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells. |
| 221731 | pcDNA3-FLAG3-drAgo2D-E | Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells. |
| 221732 | pcDNA3-FLAG3-drAgo2Y-F | Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells. |
| 221733 | pcDNA3-FLAG3-drAgo2DY-EF | Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells. |
| 221734 | pcDNA3-FLAG3-drAgo2D-A | Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells. |