On-demand biomanufacturing of protective conjugate vaccines.

Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP, Jewett MC
Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9444. doi: 10.1126/sciadv.abe9444. Print 2021 Feb. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
132780	CLM24 ∆lpxM	CLM24 strain with lpxM deletion
128404	pTrc99S-ssDsbA-EPA-DNNNS-DQNRT	Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
128403	pTrc99S-ssDsbA-CRM197-4xDQNAT	Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli
128402	pTrc99S-ssDsbA-TTlight-4xDQNAT	Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
128401	pTrc99S-ssDsbA-TTc-4xDQNAT	Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli
128400	pTrc99S-ssDsbA-PorA-4xDQNAT	Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli
128399	pTrc99S-ssDsbA-PD-4xDQNAT	Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli
128398	pTrc99S-ssDsbA-MBP-4xDQNAT	Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli
128397	pJL1-EPA-DNNNS-DQNRT	In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites
128395	pJL1-CRM197-4xDQNAT	In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites