Genetically encoded split-luciferase biosensors to measure endosome disruption rapidly in live cells.
Kilchrist KV, Tierney JW, Duvall CL
ACS Sens. 2020 Jun 23. doi: 10.1021/acssensors.0c00103.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128386 | pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394 | Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption |
| 128387 | pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394 | Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption |