amdS as a dominant recyclable marker in Cryptococcus neoformans.

Erpf PE, Stephenson CJ, Fraser JA
Fungal Genet Biol. 2019 Jun 17;131:103241. doi: 10.1016/j.fgb.2019.103241. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
128357	pPEE7	Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
128358	pPEE8	Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.
128359	pPEE13	Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.
128360	pPEE16	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.
128361	pPEE17	Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
128362	pPEE19	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the amdS2 counterselectable marker.
128363	pPEE20	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the amdS2 counterselectable marker.
128364	pPEE21	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the amdS2 counterselectable marker.
128365	pPEE22	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the amdS2 counterselectable marker.
128366	pPEE23	Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the amdS2 counterselectable marker.
128367	pPEE27	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128368	pPEE28	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128369	pPEE29	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128370	pPEE30	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128371	pPEE31	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
128372	pPEE32	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the NEO resistance marker
128373	pPEE33	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the NEO resistance marker
128374	pPEE34	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the NEO resistance marker
128375	pPEE35	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the NEO resistance marker
128376	pPEE36	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the NEO resistance marker
128377	pPEE37	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the HYG resistance marker.
128378	pPEE38	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 4, a small gene-free region. Contains the HYG resistance marker.
128379	pPEE39	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 5, a small gene-free region. Contains the HYG resistance marker.
128380	pPEE40	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 6, a small gene-free region. Contains the HYG resistance marker.
128381	pPEE41	Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the HYG resistance marker.