C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2.
Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, Nwosu J, Katselis G, Wu Y
Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128306 | pET29a-hNABP1 | Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag |
| 128307 | pET29a-hNABP2 | Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag |
| 128308 | pET29a-hNABP2-N | Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag |
| 128309 | pET29a-hNABP2-C | Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag |
| 128310 | pET29a-hNABP1-N | Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag |
| 128311 | pET29a-hNABP1-C | Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag |
| 128312 | pET29a-hNABP1NhNABP2C | Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag |
| 128313 | pET29a-hNABP2NhNABP1C | Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag |