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Noise-reducing optogenetic negative-feedback gene circuits in human cells.

Guinn MT, Balazsi G
Nucleic Acids Res. 2019 Jul 3. pii: 5527975. doi: 10.1093/nar/gkz556. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128270pMTG01-Amp-FRTPlasmid that produces the following fusion protein: TetR-LOV-TIP
128271pMTG02-Amp-FRTPlasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)
128272pMTG03-AmpFRTTIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFP
128273pMTG04-AmpFRTDeg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFP
128274pMTG05-Amp-FRTTIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFP
128275pMTG06-Amp-FRTDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFP
128276pMTG07-Amp-FRTGene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65
128277pMTG8-Amp-FRTTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP

Antibodies from Article