Unintended perturbation of protein function using GFP nanobodies in human cells.
Kuey C, Larocque G, Clarke NI, Royle SJ
J Cell Sci. 2019 Nov 6;132(21). pii: jcs.234955. doi: 10.1242/jcs.234955.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128267 | pMito-mCherry-FRB_IRES_FKBP(I)-GBPen | Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain). |
| 128268 | pMito-mCherry-FRB_IRES_FKBP(III)-GBPen | Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains). |
| 128269 | pMito-GBPen | Expression of mitochondrially targeted GBPen (GFP Nanobody) |