Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides.

Schultz JC, Cao M, Zhao H
Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
128177	NM9-SpCas9-NLS3	Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter
128178	NM1-5S-tRNA-SgH	Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter