Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing.
Tapscott T, Guarnieri MT, Henard CA
Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128159 | pCAH01SpR | pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS |
| 128160 | pCAH01Sp::Cas9 | Ptet-controlled expression of S. pyogenes Cas9 |
| 128161 | pCAH01Sp::Cas9D10A | Ptet-controlled expression of S. pyogenes Cas9D10A variant |
| 164160 | pQCH | IncQ BHR plasmid |