Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing.

Tapscott T, Guarnieri MT, Henard CA
Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
164160	pQCH	IncQ BHR plasmid
128161	pCAH01Sp::Cas9D10A	Ptet-controlled expression of S. pyogenes Cas9D10A variant
128160	pCAH01Sp::Cas9	Ptet-controlled expression of S. pyogenes Cas9
128159	pCAH01SpR	pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS