Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts.

Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 10.1038/s41592-019-0508-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
128407	pCI108-SiT-Cas12a-[Cond]	Conditionally express both AsCas12a and CRISPR arrays on single transcripts
128406	pCI118-SiT-Cas12a-[Ind]	Inducible expression of both AsCas12a and CRISPR arrays on single transcripts
128405	pCI152-pLenti-SiT-Cas12a	Express both AsCas12a and CRISPR arrays on single transcripts
128136	pCE059-SiT-Cas12a-[Activ]	Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript
128132	pCE046-SiT-Cas12a-[Repr]	Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript
128124	pCE048-SiT-Cas12a	Express both AsCas12a and CRISPR arrays on single transcripts