Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts.
Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 10.1038/s41592-019-0508-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128124 | pCE048-SiT-Cas12a | Express both AsCas12a and CRISPR arrays on single transcripts |
| 128132 | pCE046-SiT-Cas12a-[Repr] | Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript |
| 128136 | pCE059-SiT-Cas12a-[Activ] | Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript |
| 128405 | pCI152-pLenti-SiT-Cas12a | Express both AsCas12a and CRISPR arrays on single transcripts |
| 128406 | pCI118-SiT-Cas12a-[Ind] | Inducible expression of both AsCas12a and CRISPR arrays on single transcripts |
| 128407 | pCI108-SiT-Cas12a-[Cond] | Conditionally express both AsCas12a and CRISPR arrays on single transcripts |