Metabolic perceptrons for neural computing in biological systems.
Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, Faulon JL
Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128125 | pSB1K3_pBen-sfGFP_J23101-BenR | Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032 |
| 128126 | pSB1K3_pBen-sfGFP_J23101-mRFP | Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032 |
| 128127 | pSB4C5_pBen-BenR | Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop. |
| 128129 | pSB4C5_J23101-CocE | Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032 |
| 128130 | pSB4C5_J23101-vdh | Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032 |
| 128133 | pBEAST_J23101-HipO | The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032 |
| 128134 | pBEAST_J23101-vdh | The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032 |
| 128135 | pBEAST_J23101-amidase | The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032 |
| 128137 | pBEAST_J23101-bphC | The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032 |
| 128138 | pBEAST_J23101-bphD | The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032 |