Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity.
Aschenbrenner S, Kallenberger SM, Hoffmann MD, Huck A, Eils R, Niopek D
Sci Adv. 2020 Feb 5;6(6):eaay0187. doi: 10.1126/sciadv.aay0187. eCollection 2020 Feb.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128115 | Cas-Acr (wtAcrIIA4) | Expresses SpyCas9 fused to wild-type AcrIIA4 |
| 128116 | Cas-Acr (insertion 5) | Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5) |
| 128117 | Cas-Acr (N39A) | Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A) |
| 128118 | Cas-Acr (D14A/G38A) | Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A) |
| 128119 | sgRNA AAVS1 | Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker) |
| 128120 | sgRNA HBB | Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker) |
| 128121 | sgRNA EMX1 | Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker) |
| 128122 | sgRNA RUNX | Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker) |
| 128123 | sgRNA HEK | Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker) |