The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation.
Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J
Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub 2019 Aug 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128010 | OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast | traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites |
| 128011 | LN-entry+NLS | Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer |
| 128012 | LN-entry | Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer |
| 128013 | Gal4-entry+NLS | Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer |
| 128014 | Gal4-entry | Vector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer |