CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.
Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M
Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124229 | CLYBL-TO-hNGN2-BSD-mApple | Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette. |
| 127965 | pMK1334 | sgRNA expression vector compatible with CROP-Seq and imaging screens |
| 127966 | pMTL3 | constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus |
| 127967 | pMTL5 | DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus |
| 127968 | pC13N-dCas9-BFP-KRAB | constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab) |
| 127969 | pRT029 | double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus |