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Mark Groudine Lab: Plasmids for generating AID/Degron Cell Lines in Human and Mouse

Unpublished

Plasmids from Article

ID Plasmid Purpose
127897Mouse 3' Hp1a AID GFP PuroRHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene
127898pX330 Mouse 3' Hp1a gRNAWT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
127899Mouse 5' Npm1 AID GFP PuroRHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 Gene
127900pX330 Mouse 5' Npm1 gRNAWT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
127901Mouse 3' Ubf AID GFP PuroRHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene
127902pX330 Mouse 3' Ubf gRNAWT Cas9 Vector targeting the 3' end of the mouse Ubf gene
127903Mouse 5' Srcap AID GFP PuroHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene
127904pX335 Mouse 5' Srcap gRNA ACas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
127905pX335 Mouse 5' Srcap gRNA BCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
127908pSpCas9(BB)-2A-Blast_HP1beta_gRNAWT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

Antibodies from Article