Microhomology based CRISPR tagging tools for protein tracking, purification, and depletion.
Lin DW, Chung BP, Huang JW, Wang X, Huang L, Kaiser P
J Biol Chem. 2019 May 28. pii: RA119.008422. doi: 10.1074/jbc.RA119.008422.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127875 | pX330-BbsI-PITCh | For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly |
| 127881 | pN-PITCh-3XHA | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging |
| 127882 | pN-PITCh-HF | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging |
| 127883 | pN-PITCh-HBTH | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging |
| 127884 | pN-PITCh-H3FAID | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging |
| 127885 | pN-PITCh-FAID | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging |
| 127887 | pN-PITCh-mCherry | Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging |
| 127888 | pN-PITCh-GFP | Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging |