Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages.
Ling ITC, Sauka-Spengler T
Nat Cell Biol. 2019 Dec;21(12):1504-1517. doi: 10.1038/s41556-019-0428-9. Epub 2019 Dec 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127770 | pTK-EdnrbE2-Citrine | Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity |
| 127771 | pTK-EdnrbE2-Cerulean | Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Cerulean activity |
| 127772 | pTK-EdnrbE2-mCherry | Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity |
| 127773 | pTK-EdnrbE1-Citrine | Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity |
| 127775 | pTK-EdnrbE2-Sox10-FLAG-tev-AVI | Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag |
| 127776 | pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine | Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus |
| 127777 | pU6-Sox3gRNA-T1 | Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359) |
| 127778 | pU6-Sox3gRNA-T2 | Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359) |
| 127779 | pTK-RetE1-Citrine | Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity |
| 127781 | pCI-NLS-BirA-2A-mCherry | BirA construct in a PCI nls backbone driving mCherry activity |