VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells.
English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh D, Strachan RT, Wacker D, Roth BL
Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127690 | pcDNA3.1 5HT2A_NB2_1 | Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A. |
| 127691 | pTREX GPR68_NB2_2 | Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68. |
| 127692 | pSRE SSG | Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence. |
| 127693 | pSin Capsid | Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence. |
| 127694 | pSin Helper | Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence. |
| 127695 | pTSin EGFP | Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence |