LADL: light-activated dynamic looping for endogenous gene expression control.
Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE
Nat Methods. 2019 Jun 24. pii: 10.1038/s41592-019-0436-5. doi: 10.1038/s41592-019-0436-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127664 | LADL Anchor | Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter |
| 127665 | Empty Anchor Control | Encodes for puromycin resistance expressed from the EF1a promoter |
| 127666 | Empty Bridge Control Zfp462-Klf4SE | Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer. |
| 127667 | Empty Target Control | Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter |
| 127668 | LADL Bridge + Target Zfp462-Klf4SE | Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters |
| 127669 | LADL Bridge + Promoter Only Target | Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters |
| 127670 | S12.1_Empty_Bridge | Ampicillin resistant multiplexing backbone without CRY2HA and mCherry |
| 127671 | S12.2_Bridge + Target | Ampicillin resistant multiplexing backbone with CRY2HA and mCherry |