LADL: light-activated dynamic looping for endogenous gene expression control.

Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE
Nat Methods. 2019 Jun 24. pii: 10.1038/s41592-019-0436-5. doi: 10.1038/s41592-019-0436-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
127671	S12.2_Bridge + Target	Ampicillin resistant multiplexing backbone with CRY2HA and mCherry
127670	S12.1_Empty_Bridge	Ampicillin resistant multiplexing backbone without CRY2HA and mCherry
127669	LADL Bridge + Promoter Only Target	Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
127668	LADL Bridge + Target Zfp462-Klf4SE	Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
127667	Empty Target Control	Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter
127666	Empty Bridge Control Zfp462-Klf4SE	Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.
127665	Empty Anchor Control	Encodes for puromycin resistance expressed from the EF1a promoter
127664	LADL Anchor	Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter