rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP
Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133893 | pAW | Expression of Gal4AD-Prey hybrid protein |
| 133894 | pAWH | Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H |
| 133895 | pAWH-largeT | Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB |
| 133896 | pAWH-IRP | Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H |
| 133897 | pBW | Expression of Gal4BD-Bait hybrid protein. |
| 133898 | pBKWH | Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH |
| 133899 | pBKWH-Lam | Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH |
| 133900 | pBKWH-p53 | Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH |
| 133901 | pBSWHhc | Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH |
| 133902 | pBSWHhc-Lam | Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH |
| 133903 | pBSWHhc-p53 | Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH |
| 133904 | pMS22H | Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH |
| 133905 | pMS22H-IRE | Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH |
| 136403 | pHTNW | Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay. |
| 136404 | pNLF1W | Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay. |