High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq).
Andrews SS, Schaefer-Ramadan S, Al-Thani NM, Ahmed I, Mohamoud YA, Malek JA
J Biol Chem. 2019 Jul 26;294(30):11549-11558. doi: 10.1074/jbc.RA119.008792. Epub 2019 Jun 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127462 | pBORF-AD | ORF filtering plasmid (RNAp associated plasmid for pAVA) |
| 127463 | pBORF-DBD | ORF filtering plasmid (lambda cI associated plasmid for pAVA) |
| 127464 | pAVA | AVA-seq plasmid with duel BstXI sites |
| 127480 | pAVA-Gal11p-LGF2 | Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction |
| 127481 | pAVA-LGF2-Gal11p | Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction |
| 127482 | pAVA-Gal11p-LGF2(fs) | Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction |
| 127483 | pAVA-LGF2(fs)-Gal11p(fs) | Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction |