An amber obligate active site-directed ligand evolution technique for phage display.
Tharp JM, Hampton JT, Reed CA, Ehnbom A, Chen PC, Morse JS, Kurra Y, Perez LM, Xu S, Liu WR
Nat Commun. 2020 Mar 13;11(1):1392. doi: 10.1038/s41467-020-15057-7.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
127445 | pEDF-PhdRS | Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage |