Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization.
Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127396 | pFUGW Cas9-BFP | Cas9 expression |
| 127397 | library backbone 3'LTR gRNA and barcode | Digestion by BstXI and BamHI to release the backbone |