Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors.
Kearse MG, Goldman DH, Choi J, Nwaezeapu C, Liang D, Green KM, Goldstrohm AC, Todd PK, Green R, Wilusz JE
Genes Dev. 2019 Jun 6. pii: gad.324715.119. doi: 10.1101/gad.324715.119.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127299 | pcDNA3.1(+)/AUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon |
| 127300 | pcDNA3.1(+)/CUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon |
| 127301 | pcDNA3.1(+)/GUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon |
| 127302 | pcDNA3.1(+)/UUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon |
| 127303 | pcDNA3.1(+)/ACG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon |
| 127304 | pcDNA3.1(+)/AGG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon |
| 127305 | pcDNA3.1(+)/AUU-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon |
| 127306 | pcDNA3.1(+)/AAA-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid) |
| 127307 | pcDNA3.1(+)/GGG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid) |
| 127308 | pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon |
| 127309 | pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon |
| 127310 | pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon |
| 127311 | pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon |
| 127312 | pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST | Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon |
| 127313 | pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST | Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon |
| 127314 | pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST | Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon |
| 127315 | pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon |
| 127316 | pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon |
| 127317 | pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon |
| 127318 | pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon |
| 127319 | pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon |
| 127320 | pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid) |
| 127321 | pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid) |
| 127322 | pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR |
| 127323 | pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR |
| 127324 | pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR |
| 127325 | pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR |
| 127326 | pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR |
| 127327 | pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR |
| 127328 | pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR |
| 127329 | pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG | Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR |
| 127330 | pcDNA3.1(+)/GUG-DAP5-3XFLAG | Express 3xFLAG tagged DAP5 protein from GUG start codon |
| 127331 | pcDNA3.1(+)/AUG-DAP5-3XFLAG | Express 3xFLAG tagged DAP5 protein from AUG start codon |
| 127332 | pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST | Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES |
| 127333 | pGL4.13/AUG-FFLuc-3XFLAG | Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon |
| 127334 | pGL4.13/GUG-FFLuc-3XFLAG | Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon |
| 127335 | pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG | Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA) |
| 127336 | pGL4.13/AUG(30)-FFLuc-3XFLAG | Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30 |
| 127337 | pGL4.13/AUG(59)-FFLuc-3XFLAG | Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59 |