Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics.
Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH
Nat Methods. 2019 Oct 14. pii: 10.1038/s41592-019-0583-8. doi: 10.1038/s41592-019-0583-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127237 | pAAV-hSyn-ChRger2-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter. |
| 127238 | pAAV-CaMKIIa-ChRger2-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter. |
| 127239 | pAAV-CAG-DIO(ChRger2-TS-YFP) | High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed. |
| 127240 | pAAV-hSyn-ChRger3-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter. |
| 127241 | pAAV-CaMKIIa-ChRger3-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter. |
| 127242 | pAAV-CAG-DIO(ChRger3-TS-YFP) | High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed. |
| 127243 | pAAV-hSyn-ChRger1-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter. |
| 127244 | pAAV-CaMKIIa-ChRger1-TS-EYFP | High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter. |
| 127245 | pAAV-CAG-DIO(ChRger1-TS-YFP) | High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed. |
| 129393 | pAAV-CaMKII-STChRger2-TS-EYFP | Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter. |
| 129394 | pAAV-CAG-DIO-(STChRger2-TS-EYFP) | Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed. |
| 129395 | pAAV-hSyn-STChRger2-TS-EYFP | Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter. |