Plant gene editing through de novo induction of meristems.

Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 10.1038/s41587-019-0337-2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
127210	pMM113	BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS
127211	pMM114	BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS
127212	pMM117	BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS
127213	pMM119	BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS
127214	pMM131	BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS
127215	pMM134	BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS
127216	pMM135	BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS
127217	pMM136	BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS
127218	pMM146	BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS
127219	pMOD_C'5014	Module C' with Pnos::WUS2
127220	pMOD_D4910	Module D with ZmUbi1::BBM
127221	pRN114	BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM
127222	pRN227	BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS
127223	pMOD_A5801	35S::Luciferase
127224	pMOD_A5803	CmYLCV::Luciferase
127225	pMM100	AtU6::sgRNA targeting PDS
127226	pMOD_C'0000a	Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.
127227	pMM107	35S::IPT::pinII terminator
127228	pRN110	CmYLCV::STM::35S terminator
127229	pMM112	35S::IPT::pinII terminator
127230	pMOD_D0000a	Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.
127231	pRN104	35S::AFR5::35S terminator
133312	pMKV057	BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT
133313	pMKV058	BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2
133314	pMKV059	BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT
133315	pMKV060	BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+
160696	pRN120	BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator