Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.
Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T, Johnsson K
ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 10.1021/acssensors.7b00331. Epub 2017 Aug 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127180 | pET51b(+)-SNAP-CLIP | Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag |
| 127181 | pET51b(+)-SNAP-HCA_sensor | Bacterial expression of the fusion protein used for sulfa-drugs sensing |
| 127182 | pEBTet-SNAP-HCA_sensor | Eukaryotic expression of the fusion protein used for sulfa-drugs sensing |
| 127184 | pEBTet-p52-HDM2_sensor | Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction |