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De novo design of bioactive protein switches.

Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, El-Samad H, Baker D
Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 10.1038/s41586-019-1432-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
127200pAN1578Progesterone inducible expression of KeyA-BFP
127201pAN1579Progesterone inducible expression of KeyC-BFP
127202pAN1572Dual inducible expression of Venus-degronSwitchA and KeyA-BFP
127203pAN1935Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC
127204pAN1735Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS
127205pAN1646pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
127206pAN1777Dual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLS
127246pAN2981Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

Antibodies from Article