Optical Pooled Screens in Human Cells.
Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC
Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127168 | LentiGuide-BC-start | Entry plasmid for two-step guide-barcode cloning |
| 127169 | LentiGuide-BC-CMV-Puro | PCR template for CMV-puro resistance cassette |
| 127170 | LentiGuide-BC-EF1a-Puro | PCR template for EF1a-puro resistance cassette |
| 127171 | pL_FR_Hygro | Frameshift reporter (control plasmid for screening) |
| 127172 | p65-mNeonGreen | Fluorescently tagged p65 reporter |
| 127173 | CROPseq-Guide-Zeo | Entry plasmid for guide cloning |
| 127458 | CROPseq-puro-v2 | Entry plasmid for guide cloning |