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Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies.

Rajendran PS, Challis RC, Fowlkes CC, Hanna P, Tompkins JD, Jordan MC, Hiyari S, Gabris-Weber BA, Greenbaum A, Chan KY, Deverman BE, Munzberg H, Ardell JL, Salama G, Gradinaru V, Shivkumar K
Nat Commun. 2019 Apr 26;10(1):1944. doi: 10.1038/s41467-019-09770-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
127090pAAV-CAG-DIO-ChR2(H134R)-eYFPCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
127091pAAV-CAG-DIO-tTAAn AAV genome with Cre-dependent expression of tTA from the CAG promoter
127092pAAV-TRE-DIO-tdTomato-fAn AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Antibodies from Article