Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing.
Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127247 | IgH intron enhancer | To maximize gene expression in B cells |
| 127248 | MPC11 IgG2b gene | Expresses intact IgG2b gene in mammalian cells |
| 127250 | Dom neg CstF64 | Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation |
| 127252 | NH2end ELL2 | Expresses the N terminal segment of human ELL2 in mammalian cells |
| 127253 | Full human ELL2 | Expresses full-length human ELL2 in mammalian cells |
| 127254 | Human/mouse ELL2 | Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells |
| 127255 | COOHend ELL2 | Expresses the C terminal segment of human ELL2 in mammalian cells |
| 127256 | ELL2mRNA+polyA | For in vitro translation of human ELL2 with 30 nt pA tail |
| 127257 | frameshift ELL2 | For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation. |
| 127258 | Met1ILeu ELL2 | For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation |
| 127259 | Met133ILeu ELL2 | For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation |
| 127260 | Met138ILeu ELL2 | For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation |
| 127261 | Met186ILeu ELL2 | For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation |
| 127262 | Met331Leu ELL2 | For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation |
| 127263 | Met133I, M1381I, M186I ELL2 | For in vitro translation of human ELL2 with Met133I, M1381I, M186I |
| 127264 | Met133ILeu, Met186ILeu ELL2 | For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu |
| 127265 | Met138ILeu, Met186ILeu ELL2 | For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu |
| 127266 | 289aa ELL2 | For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations. |
| 127267 | 564aa ELL2 bluescript | For in vitro translation of human ELL2 lacking COOH region |
| 127268 | HA tagged Xba ELL2 | For in vitro translation of WT ELL2 with HA tag inserted near N-term |
| 127269 | HA tagged Xba ELL2 Met133, 138, 186 to Ileu | For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu |
| 127270 | HA tagged Sph ELL2 | For in vitro translation of human WT ELL2 with internal HA tag |
| 127271 | HA tagged Sph ELL2 Met133, 138, 186 to Ileu | For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met |
| 127272 | 289aa Met133, 138, 186Ileu ELL2 | Expresses 289aa human ELL2 with Met133, 138, 186Ileu |
| 127273 | 563aa ELL2 pEF4B | Expresses 563aa human WT ELL2 |
| 127274 | HA tagged mid ELL2 | Expresses human ELL2 with HA tag in the middle that does not disrupt function |
| 127276 | HA tagged mid ELL2 M133, 138, 186ILeu | Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function |
| 127277 | HA tagged SphI ELL2 M133, 138, 186ILeu | Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminus |