Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology.
Valenzuela-Ortega M, French C
Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 10.1093/synbio/ysab003. eCollection 2021.
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PubMed
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Article
Joint Universal Modular Plasmids (JUMP) is a universal vector for Modular Cloning (Golden Gate) assembly. JUMP is a flexible platform based on Standard European Vector Architecture (SEVA), compatible with PhytoBricks and BioBricks (and the Registry of Standard Biological Parts). The toolkit includes vectors with four selection markers, ten origins of replication (integrative and episomal with diverse copy number), and a collection of microbial basic parts. To allow tailored modification, all vectors can be modified using modular cloning in an orthogonal way using multiple cloning sites. This allows researchers to introduce any feature into any JUMP vector and simplifies the Design-Build-Test cycle of synthetic biology.
Please see the Supplementary Information in the bioRxiv preprint for detailed methodologies.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126956 | pJUMP19-min2 | "Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors. |
| 126957 | pJUMP19-Pac | Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI. |
| 126958 | pJUMP19-Tac | Terminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI. |
| 126959 | pJUMP18-Uac | Universal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8. |
| 126960 | pJUMP29-1A(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126961 | pJUMP29-1B(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126962 | pJUMP29-1C(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126963 | pJUMP29-1D(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126964 | pJUMP49-2A(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126965 | pJUMP49-2B(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126966 | pJUMP49-2C(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126967 | pJUMP49-2D(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126968 | pJUMP21-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin R6K (pir conditional). |
| 126969 | pJUMP22x-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites. |
| 126970 | pJUMP23-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range) |
| 126971 | pJUMP24-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 126972 | pJUMP25-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range) |
| 126973 | pJUMP26-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin p15A (medium copy number) |
| 126974 | pJUMP27-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number) |
| 126975 | pJUMP27ts-1A(sfGFP) | Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number) |
| 126976 | pJUMP28-1A(sfGFP) | Level 1 vector. sfGFP reporter. Origin pUC (high copy number) |
| 126977 | pJUMP27ts-1A(lacZ) | Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number) |
| 126978 | pJUMP29-1A(lacZ) | Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number). |
| 126979 | pJUMP29[dCas9]-1A(lacZ) | Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site. |
| 126980 | pJUMP27ts[mCherry]-1A(lacZ) | Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site. |
| 126981 | pJUMP29-1D'(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126982 | pJUMP29-1E(sfGFP) | Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126983 | pJUMP39-1A(sfGFP) | Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126984 | pJUMP38-1A(sfGFP) | Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter. |
| 126985 | pJUMP41-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin R6K (pir conditional). |
| 126986 | pJUMP42x-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites. |
| 126987 | pJUMP43-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range) |
| 126988 | pJUMP44-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle) |
| 126989 | pJUMP45-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range) |
| 126990 | pJUMP46-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin p15A (medium copy number) |
| 126991 | pJUMP47-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number) |
| 126992 | pJUMP47ts-2A(sfGFP) | Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number) |
| 126993 | pJUMP48-2A(sfGFP) | Level 2 vector. sfGFP reporter. Origin pUC (high copy number) |
| 126994 | pJUMP49-2D'(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126995 | pJUMP49-2E(sfGFP) | Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter. |
| 126996 | pJUMP19-J23100_P | Basic Part P- Promoter (constitutive); J23100 |
| 126997 | pJUMP19-J23102_P | Basic Part P- Promoter (constitutive); J23102. |
| 126998 | pJUMP19-J23103_P | Basic Part P- Promoter (constitutive); J23103. |
| 126999 | pJUMP19-J23106_P | Basic Part P- Promoter (constitutive); J23106. |
| 127000 | pJUMP19-J23107_P | Basic Part P- Promoter (constitutive); J23107. |
| 127001 | pJUMP19-J23119s_P | Basic Part P- Promoter (constitutive); J23119 (1bp short on 3'). |
| 127002 | pJUMP19-T7pro_P | Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase |
| 127003 | pJUMP19-Pbad_P | Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC. |
| 127004 | pJUMP19-Ptac_P | Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI. |
| 127005 | pJUMP19-Ptet_P | Basic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetR |
| 127006 | pJUMP19-Pci_P | Basic Part P- Promoter (non-constitutive); Pci; controlled by cI. |
| 127007 | pJUMP19-Pars_P | Basic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR |
| 127008 | pJUMP19-Pmer_P | Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR |
| 127009 | pJUMP19-Pcue_P | Basic Part P- Promoter (non-constitutive); Pcue; metal sensitive; controlled by CueR |
| 127010 | pJUMP18-B0032_R | Basic Part R- RBS; B0032m Ribosome Binding Site. |
| 127011 | pJUMP18-B0033_R | Basic Part R- RBS; B0033m Ribosome Binding Site. |
| 127012 | pJUMP18-B0034_R | Basic Part R- RBS; B0034m Ribosome Binding Site. |
| 127013 | pJUMP18-RBS-pET_R | Basic Part R- RBS; pET vector Ribosome Binding Site. |
| 127014 | pJUMP18-RBS-pET-MV_RN | Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start. |
| 127015 | pJUMP18-B0032-MV_RN | Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start. |
| 127016 | pJUMP18-B0033-MV_RN | Basic Part RN- RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus start. |
| 127017 | pJUMP18-B0034-MV_RN | Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start. |
| 127018 | pJUMP18-sfGFP_O | Basic Part O- ORF; sfGFP; fluorescent protein |
| 127019 | pJUMP18-amilCP_O | Basic Part O- ORF; amilCP; blue chromoprotein |
| 127020 | pJUMP18-araC_O | Basic Part O- ORF; araC; transcription factor |
| 127021 | pJUMP18-tetR_O | Basic Part O- ORF; TetR; transcription factor |
| 127022 | pJUMP18-arsR_O | Basic Part O- ORF; arsR; transcription factor |
| 127023 | pJUMP18-merR_O | Basic Part O- ORF; merR; transcription factor |
| 127024 | pJUMP18-lacI_O | Basic Part O- ORF; lacI; transcription factor |
| 127025 | pJUMP18-mCherry_O | Basic Part O- ORF; mCherry; fluorescent protein |
| 127026 | pJUMP18-cueR_O | Basic Part O- ORF; cueR; transcription factor |
| 127027 | pJUMP18-lacZ_O | Basic Part O- ORF; lacZ alpha-peptide; blue/white screening. |
| 127028 | pJUMP18-dCas9_O | Basic Part O- ORF; Catalytically dead mutant of the Cas9 from Streptococcus pyogenes. |
| 127029 | pJUMP18-cI_O | Basic Part O- ORF; cI; transcription factor from lambda phage. |
| 127030 | pJUMP18-cI-ts_O | Basic Part O- ORF; cI; transcription factor from lambda phage; thermosensitive. |
| 127031 | pJUMP18-pheS_NOC | Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase. |
| 127032 | pJUMP18-lambdaRED_NOC | Basic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS). |
| 127033 | pJUMP18-Histag_N | Basic Part N- N-terminus; polyhistidine tag |
| 127034 | pJUMP18-Streptag_N | Basic Part N- N-terminus; Strep-tag II |
| 127035 | pJUMP18-Histag_C | Basic Part C- C-terminus; polyhistidine tag |
| 127036 | pJUMP18-Streptag_C | Basic Part C- C-terminus; Strep-tag II |
| 127037 | pJUMP19-L3S1P51_T | Basic Part T- Terminator; L3S1P51; synthetic terminator |
| 127038 | pJUMP19-B0015_T | Basic Part T- Terminator; B0015. Terminator. |
| 127039 | pJUMP19-L3S2P21_T | Basic Part T- Terminator; L3S2P21; synthetic terminator |
| 127040 | pJUMP19-L3S1P32_T | Basic Part T- Terminator; L3S1P32; synthetic terminator |
| 127041 | pJUMP19-L3S1P11_T | Basic Part T- Terminator; L3S1P11; synthetic terminator |
| 127042 | pJUMP19-L2U2H09_T | Basic Part T- Terminator; L2U2H09; synthetic terminator |
| 127043 | pJUMP19-L1U1H09_T | Basic Part T- Terminator; L1U1H09; synthetic terminator |
| 127044 | pJUMP19-L1U1H08_T | Basic Part T- Terminator; L1U1H08; synthetic terminator |
| 127045 | pJUMP19-B0015_CT | Basic Part CT- C-terminus + Terminator; B0015. C-termini STOP + Terminator. |
| 127046 | pJUMP19-L3S2P21_CT | Basic Part CT- C-terminus + Terminator; L3S2P21. C-termini STOP + terminator (synthetic) |
| 127047 | pJUMP19-L3S1P51_CT | Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic) |
| 127048 | pJUMP19-L2U2H09_CT | Basic Part CT- C-terminus + Terminator; L2U2H09. C-termini STOP + terminator (synthetic) |
| 127049 | pJUMP19-L1U1H09_CT | Basic Part CT- C-terminus + Terminator; L1U1H09. C-termini STOP + terminator (synthetic) |
| 127050 | pJUMP19-L1U1H08_CT | Basic Part CT- C-terminus + Terminator; L1U1H08. C-termini STOP + terminator (synthetic) |
| 127051 | pJUMP18-cat_TU | Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61. |