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The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length.

Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS
Mol Biol Cell. 2018 Apr 15;29(8):886-896. doi: 10.1091/mbc.E17-12-0729. Epub 2018 Mar 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
126874IDA3::HA exon2 (WS13)p3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.
126875IDA3::NeonGreen N-term (WS14)NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.
126876IDA3::NeonGreen exon2 (WS15)NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

Antibodies from Article