Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies.
Lee KZ, Mechikoff MA, Parasa MK, Rankin TJ, Pandolfi P, Fitzgerald KS, Hillman ET, Solomon KV
ACS Synth Biol. 2022 Jan 21;11(1):53-60. doi: 10.1021/acssynbio.1c00340. Epub 2022 Jan 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179578 | pColE1-ISceI | Expresses I-SceI from an arabinose promoter |
| 179579 | pFREE2-xCas9-sgRNA | Expresses xCas9 and gRNA that targets ColE1 plasmids for curing. |
| 179580 | pFREE2-eSpCas9-sgRNA | Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing |
| 179582 | pFREE2-SpCas9-sgRNA | Expresses spCas9 and gRNA that targets ColE1 plasmids for curing |