Single-Cell Transcriptomic Profiling of Pluripotent Stem Cell-Derived SCGB3A2+ Airway Epithelium.
McCauley KB, Alysandratos KD, Jacob A, Hawkins F, Caballero IS, Vedaie M, Yang W, Slovik KJ, Morley M, Carraro G, Kook S, Guttentag SH, Stripp BR, Morrisey EE, Kotton DN
Stem Cell Reports. 2018 May 8;10(5):1579-1595. doi: 10.1016/j.stemcr.2018.03.013. Epub 2018 Apr 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126697 | pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR | donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence |
| 126698 | pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP | to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence |