Enhanced Cas12a editing in mammalian cells and zebrafish.
Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA
Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120801 | pET-21a_2xNLS_FnCpf1_MBP_protein_expression | Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone |
| 124548 | pET-21a_FnCpf1_2xNLS_protein_expression | Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone |
| 126637 | AsCas12a-2C-NLS in pCSDest | Expresses AsCas12a-2C-NLS in mammalian cells |
| 126638 | LbCas12a-2C-NLS in pCSDest | Expresses LbCas12a-2C-NLS in mammalian cell |
| 126639 | FnoCas12a-2C-NLS in pCSDest | Expresses FnoCas12a-2C-NLS in mammalian cells |
| 126640 | AsCas12a-DR-cr in PBCSK- | Cloning vector AsCas12a DR-crRNA for expression in mammalian cells |
| 126641 | LbCas12a-DR-cr in PBCSK- | Cloning vector LbCas12a DR-crRNA for expression in mammalian cells |
| 126642 | FnoCas12a-DR-cr in PBCSK- | Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells |
| 126643 | LbCas12a-DR-GC-swap-cr in pBCSK+ | Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells |
| 126644 | FnoCas12a-DR-GC-swap-cr in pBCSK+ | Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells |