Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic memory in a context-dependent manner.
O'Geen H, Bates SL, Carter SS, Nisson KA, Halmai J, Fink KD, Rhie SK, Farnham PJ, Segal DJ
Epigenetics Chromatin. 2019 May 3;12(1):26. doi: 10.1186/s13072-019-0275-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126587 | DNMT3L-dCas9 | DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 126588 | dCas9-DNMT3L | DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression |
| 126589 | NLS-HA-2xMCP-KRAB | Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone |
| 126590 | NLS-HA-2xMCP-Ezh2 | Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone |