Lineage tracing and analog recording in mammalian cells by single-site DNA writing.

Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
126424	pcDNA-Cas9-T2A-TdT	Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)
126425	pcDNA-Cas9-T2A-STOP-TdT	Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
126426	pTBL515 AA sgRNA	To target a protospacer with AA at the cut site.
126427	pTBL402 AC sgRNA	To target a protospacer with AC at the cut site.
126428	pTBL071 AG sgRNA	To target a protospacer with AG at the cut site.
126429	pTBL509 AT sgRNA	To target a protospacer with AT at the cut site.
126430	pTBL510 CA sgRNA	To target a protospacer with CA at the cut site.
126431	pTBL511 CC sgRNA	To target a protospacer with CC at the cut site.
126432	pTBL512 CG sgRNA	To target a protospacer with CG at the cut site.
126433	pTBL516 CT sgRNA	To target a protospacer with CT at the cut site.
126434	pTBL507 GA sgRNA	To target a protospacer with GA at the cut site.
126435	pTBL401 GC sgRNA	To target a protospacer with GC at the cut site.
126436	pTBL251 GG sgRNA	To target a protospacer with GG at the cut site.
126437	pTBL063 GT sgRNA	To target a protospacer with GT at the cut site.
126438	pTBL508 TA sgRNA	To target a protospacer with TA at the cut site.
126439	pTBL250 TC sgRNA	To target a protospacer with TC at the cut site.
126440	pTBL514 TG sgRNA	To target a protospacer with TG at the cut site.
126441	pTBL513 TT sgRNA	To target a protospacer with TT at the cut site.
126442	pTBL405 CHYRON1 integration construct	To integrate the CHYRON1 locus at HEK293site3.
126445	pTBL436 HEK293site3EQR sgRNA	To cut the human HEK293 site3 locus for integration.
126446	pTBL649 CHYRON2 integration construct	To integrate the CHYRON2 locus at AAVS1 in human cells.
126447	pTBL437 AAVS1 EQR sgRNA	To cut the human AAVS1 locus for integration.
126448	pTBL680 CHYRON3 integration construct	To integrate the CHYRON3 locus at AAVS1 in human cells.
126449	pTBL681 CHYRON4 integration construct	To integrate the CHYRON4 locus at AAVS1 in human cells.
126450	pcDNA-TdT	Expresses human TdT in mammalian cells. (pTBL206)
126451	pTBL210	Expresses Cas9-XTEN-TdT in mammalian cells.
126454	pJHG003	Expresses Cas9-XTEN-dTdT in mammalian cells.
126455	pJHG004	Expresses dTdT in mammalian cells.
126457	pTBL472	Expresses Cas9-10xFlag-TdT in mammalian cells.
126458	pTBL469	Expresses Cas9-15xFlag-TdT in mammalian cells.
126477	pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI	Expresses Cas9-5xFlag-TdT in mammalian cells.
126480	pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI	Expresses Cas9-GSAlinker-TdT in mammalian cells.
126950	pTBL322 GC	To target a protospacer with GC at the cut site.
126951	pTBL581 GA	To target a protospacer with GA at the cut site.
126952	pTBL601 AG	To target a protospacer with AG at the cut site.
126953	pTBL602 CG	To target a protospacer with CG at the cut site.
132667	pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT	Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.
163643	pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT	Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
163644	pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9	Makes a lentivirus that expresses mNeonGreen and Cas9.