Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins.
Lee J, Mou H, Ibraheim R, Liang SQ, Liu P, Xue W, Sontheimer EJ
RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126202 | p1146-pCSDest-AcrIIC3Nme-mCherry | Mammalian expression of AcrIIC3Nme fused to mCherry |
| 126203 | p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS | Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR |
| 126204 | p1148-pCSDest-AcrIIA4-mCherry | Mammalian expression of AcrIIA4 fused to mCherry |
| 126205 | p1149-pCSDest-AcrIIA4-mCherry-miR122BS | Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR |
| 129530 | p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS | Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme |
| 129531 | p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS | Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR |
| 129532 | p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS | Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4 |
| 129534 | p1195-pssAAV.U1a.hNme2Cas9 | AAV vector expressing Nme2Cas9 |