A piggyBac-based toolkit for inducible genome editing in mammalian cells.
Schertzer MD, Thulson E, Braceros KCA, Lee DM, Hinkle ER, Murphy RM, Kim SO, Vitucci EM, Calabrese JM
RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126028 | PB_rtTA_BsmBI | PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning |
| 126029 | PB_tre_Cas9 | PiggyBac cargo vector expressing doxycycline inducible Cas9 |
| 126030 | PB_tre_dCas9_KRAB | PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion |
| 126031 | PB_tre_dCas9_VP160 | PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion |