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Light-Induced Protein Clustering for Optogenetic Interference and Protein Interaction Analysis in Drosophila S2 Cells.

Osswald M, Santos AF, Morais-de-Sa E
Biomolecules. 2019 Feb 12;9(2). pii: biom9020061. doi: 10.3390/biom9020061. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
126022pHRW-CRY2-VHH(GFP)express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics
126023pHRW-CRY2olig-VHH(GFP)express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics
126024pHW-CIBN-MP-HRW-CRY2-VHH(GFP)express RFP-tagged LARIAT for optogenetic clustering
126025pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)express RFP- and mCer-tagged LARIAT for optogenetic clustering
126026pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)express RFP-tagged LARIAT variant for optogenetic clustering
126027pHW-CIBN-MP-HFW-CRY2-VHH(GFP)express Flag-tagged LARIAT for optogenetic clustering

Antibodies from Article