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Intensiometric biosensors visualize the activity of multiple small GTPases in vivo.

Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB, Heo WD
Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
180571pCMV-G-KrasddGFP sensor of Kras
180572pCMV-R-KrasddRFP sensor of Kras
180573pCMV-G-HrasddGFP sensor of Hras
180574pCMV-R-HrasddRFP sensor of Hras
180575pCMV-G-Rac1ddGFP sensor of Rac1
180576pCMV-R-Rac1ddRFP sensor of Rac1
180577pCMV-G-Cdc42ddGFP sensor of Cdc42
180578pCMV-R-Cdc42ddRFP sensor of Cdc42
180579pAAV-CAG-R-HrasddRFP sensor of Hras
180580pAAV-CAG-G-HrasddGFP sensor of Hras
180581pAAV-CAG-G-Rac1ddGFP sensor of Rac1
180582pAAV-CAG-G-Cdc42ddGFP sensor of Cdc42
180584pCMV-RA-HRas-IRES2-Lyn-iRFP682RA-Hras and Lyn-iRFP682

Antibodies from Article