Engineering Epigenetic Regulation Using Synthetic Read-Write Modules.
Park M, Patel N, Keung AJ, Khalil AS
Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134994 | pMP315 | KRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254) |
| 134996 | pMP416 | dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A) |
| 134997 | pMP472 | Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor |
| 134998 | pMP498 | Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor |
| 134999 | pMP597 | Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR |
| 135000 | pMP650 | VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR |
| 135001 | pMP926 | m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR |