Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag.
Chen B, Zou W, Xu H, Liang Y, Huang B
Nat Commun. 2018 Nov 29;9(1):5065. doi: 10.1038/s41467-018-07498-y.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164038 | BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP | Expression of dCas9-14xGFP11 in mammalian cells |
| 164039 | BC1177-pHR-SFFV-GFP(1-10)-NLS | Expression of GFP1-10 in mammalian cells |
| 164040 | BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7) | Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2 |
| 164041 | BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor) | CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene |
| 164042 | BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor) | CRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA gene |
| 164043 | BC1364-mouse U6-sgH2B | U6-driven sgRNA targeting H2B |