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Imaging pH Dynamics Simultaneously in Two Cellular Compartments Using a Ratiometric pH-Sensitive Mutant of mCherry.

Rajendran M, Claywell B, Haynes EP, Scales U, Henning CK, Tantama M
ACS Omega. 2018 Aug 31;3(8):9476-9486. doi: 10.1021/acsomega.8b00655. Epub 2018 Aug 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207867GW1-mCherryEAMammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor
207868GW1-mito-mCherryEAMitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Antibodies from Article