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Identification of SMCHD1 domains for nuclear localization, homo-dimerization, and protein cleavage.

Hiramuki Y, Tapscott SJ
Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130675pLenti-CMV-SMCHD1-Exon1-48-3xFLAGExpresses full-length human SMCHD1 with a C-terminal 3x FLAG tag
130676pLenti-CMV-SMCHD1-deltaKRMR-3xFLAGExpresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tag
130677pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAGExpresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag
130678pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAGExpresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag
130679pLenti-CMV-SMCHD1-R1868*-3xFLAGExpresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag
130680pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAGExpresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag
130681pLenti-CMV-SMCHD1-Exon1-36-3xFLAGExpresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag
130682pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAGExpresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag
130683pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAGExpresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag
130684pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAGExpresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag
130685pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAGExpresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag
130686pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAGExpresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag
130687pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAGExpresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tag
130688pLenti-CMV-SMCHD1-Exon37-48-3xFLAGExpresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag
130689pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAGExpresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag
130690pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAGExpresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag
130691pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAGExpresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Antibodies from Article