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Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.

Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S
Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136661pVAX_CBI_AcrIIA2Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136662pVAX_CBI_AcrIIA4Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136663pVAX_CBI_AcrIIA5_D1126Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136664pVAX_CBI_AcrIIA5_D4276Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136666pVAX_CBI_AcrIIA6_D1024Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136667pVAX_CBI_AcrIIA6_D1811Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
136668pVAX_CBI_AcrIIA6_DT1Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Antibodies from Article