Fine-tuning of substrate preferences of the Src-family kinase Lck revealed through a high-throughput specificity screen.
Shah NH, Lobel M, Weiss A, Kuriyan J
Elife. 2018 Mar 16;7. pii: 35190. doi: 10.7554/eLife.35190.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214233 | pET23a-H6-TEV-cSrc | Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings |
| 214234 | pET23a-H6-TEV-cAbl | Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings |