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Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling.

Verweij FJ, Bebelman MP, Jimenez CR, Garcia-Vallejo JJ, Janssen H, Neefjes J, Knol JC, de Goeij-de Haas R, Piersma SR, Baglio SR, Verhage M, Middeldorp JM, Zomer A, van Rheenen J, Coppolino MG, Hurbain I, Raposo G, Smit MJ, Toonen RFG, van Niel G, Pegtel DM
J Cell Biol. 2018 Mar 5;217(3):1129-1142. doi: 10.1083/jcb.201703206. Epub 2018 Jan 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
130901pCMV-Sport6-CD63-pHluorinExpression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.
130902pCMV-Sport6-CD63-pHujiExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.
130903pCMV-Sport6-CD81-pHluorinExpression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.
130904pCMV-Sport6-CD81-pHujiExpression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.
130905pCMV-Sport6-CD9-pHluorinExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.
130906pCMV-Sport6-CD9-pHujiExpression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

Antibodies from Article